Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Anton Nekrutenko avatar Anton Nekrutenkoorcid logoHelena Rasche avatar Helena Rascheorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoSaskia Hiltemann avatar Saskia HiltemannWilliam Durand avatar William DurandDeepti Varshney avatar Deepti VarshneyBazante Sanders avatar Bazante SandersSimon Bray avatar Simon Brayorcid logoMateo Boudet avatar Mateo Boudetorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoBérénice Batut avatar Bérénice BatutNathan Dunn avatar Nathan Dunnorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoRomane LIBOUBAN avatar Romane LIBOUBANKenji Fujihara avatar Kenji FujiharaNiall Beard avatar Niall Beardorcid logoAnna Syme avatar Anna SymeTorsten Seemann avatar Torsten Seemannorcid logoMiaomiao Zhou avatar Miaomiao ZhouTwishi Gulati avatar Twishi Gulatiorcid logoErwan Corre avatar Erwan Correorcid logoBjörn Grüning avatar Björn Grüningorcid logoDelphine Lariviere avatar Delphine LariviereGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnika Erxleben avatar Anika ErxlebenTeresa Müller avatar Teresa Müllerorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSimon Gladman avatar Simon GladmanJasper Ouwerkerk avatar Jasper OuwerkerkEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoMaria Doyle avatar Maria Doyleorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoPaul Zierep avatar Paul Ziereporcid logoLaura Leroi avatar Laura LeroiEkaterina Polkh avatar Ekaterina Polkhorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Funding

These individuals or organisations provided funding support for the development of this resource