Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoDeepti Varshney avatar Deepti Varshneyorcid logoLaura Leroi avatar Laura LeroiGildas Le Corguillé avatar Gildas Le CorguilléGuillaume Gautreau avatar Guillaume Gautreauorcid logoErwan Corre avatar Erwan CorreValentin Loux avatar Valentin Louxorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoBjörn Grüning avatar Björn Grüningorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoTeresa Müller avatar Teresa Müllerorcid logoMaria Doyle avatar Maria DoyleSimon Bray avatar Simon Brayorcid logoDelphine Lariviere avatar Delphine LariviereNathan Dunn avatar Nathan Dunnorcid logoSimon Gladman avatar Simon Gladmanorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoAnna Syme avatar Anna SymeTwishi Gulati avatar Twishi GulatiNiall Beard avatar Niall Beardorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoBérénice Batut avatar Bérénice Batutorcid logoStéphanie Robin avatar Stéphanie RobinHélène Chiapello avatar Hélène ChiapelloBazante Sanders avatar Bazante Sandersorcid logoPaul Zierep avatar Paul ZierepAnton Nekrutenko avatar Anton Nekrutenkoorcid logoAlexandre Cormier avatar Alexandre CormierEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilKenji Fujihara avatar Kenji Fujiharaorcid logoHelena Rasche avatar Helena Rascheorcid logoCristóbal Gallardo avatar Cristóbal GallardoWilliam Durand avatar William DurandTorsten Seemann avatar Torsten SeemannJasper Ouwerkerk avatar Jasper OuwerkerkEkaterina Polkh avatar Ekaterina Polkhorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoMateo Boudet avatar Mateo Boudet

Funding

These individuals or organisations provided funding support for the development of this resource