Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoMaria Doyle avatar Maria Doyleorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoDelphine Lariviere avatar Delphine LariviereSimon Bray avatar Simon Brayorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoErwan Corre avatar Erwan Correorcid logoMateo Boudet avatar Mateo Boudetorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony BretaudeauKenji Fujihara avatar Kenji Fujiharaorcid logoLaura Leroi avatar Laura LeroiNathan Dunn avatar Nathan DunnNiall Beard avatar Niall BeardEkaterina Polkh avatar Ekaterina Polkhorcid logoStéphanie Robin avatar Stéphanie RobinBazante Sanders avatar Bazante SandersTeresa Müller avatar Teresa Müllerorcid logoPaul Zierep avatar Paul ZierepWilliam Durand avatar William Durandorcid logoBérénice Batut avatar Bérénice BatutTorsten Seemann avatar Torsten SeemannAnton Nekrutenko avatar Anton NekrutenkoTwishi Gulati avatar Twishi GulatiEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilDeepti Varshney avatar Deepti VarshneyJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoBjörn Grüning avatar Björn Grüningorcid logoSimon Gladman avatar Simon Gladmanorcid logoAnna Syme avatar Anna Symeorcid logoAlexandre Cormier avatar Alexandre CormierGildas Le Corguillé avatar Gildas Le Corguilléorcid logoFelicitas Kindel avatar Felicitas Kindel

Funding

These individuals or organisations provided funding support for the development of this resource